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Practice Flashcards

Flip each card, test yourself, and read the exam hint. Pick your board below.

O-Level: Cells

O-Level Cambridge 0610
1 / 5
Question

Which structures are found in a plant cell but NOT an animal cell?

Tap to reveal
Answer

Cell wall (cellulose), chloroplasts and a large permanent vacuole.

Hint: Exam hint: never write just "vacuole" - say large permanent vacuole.
Question

Function of the mitochondria?

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Answer

Site of aerobic respiration - releases energy from glucose as ATP.

Hint: Cells that are very active (muscle, sperm) have many mitochondria.
Question

Define diffusion.

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Answer

Net movement of particles from a region of higher concentration to lower concentration, down a concentration gradient.

Hint: Always include "net movement" and "down the gradient" for full marks.
Question

Define osmosis (0610 wording).

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Answer

The net movement of water molecules from a dilute solution to a concentrated solution through a partially permeable membrane.

Hint: Say partially permeable, not semi-permeable.
Question

What is active transport?

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Answer

Movement of particles through a cell membrane from lower to higher concentration using energy from respiration.

Hint: Key words: against the gradient + energy from respiration.

O-Level: Enzymes

O-Level Cambridge 0610
1 / 4
Question

Define enzyme.

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Answer

A protein that acts as a biological catalyst, speeding up a reaction without being changed itself.

Hint: Protein + biological catalyst = 2 marks.
Question

Explain the effect of high temperature on an enzyme.

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Answer

Above the optimum the enzyme denatures: the active site changes shape so the substrate no longer fits, so the rate falls.

Hint: Never say the enzyme is killed - it is denatured.
Question

Why is enzyme activity low at low temperature?

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Answer

Molecules have less kinetic energy, so fewer successful collisions between enzyme and substrate occur.

Question

What does pH do to enzymes?

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Answer

Each enzyme has an optimum pH; away from it the active site is denatured and the rate decreases.

Hint: Pepsin pH2, amylase pH7 - learn examples.

AS: Cell Structure

AS-Level Cambridge 9700
1 / 5
Question

State the formula for magnification.

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Answer

Magnification = image size / actual size.

Hint: Convert units first: 1 mm = 1000 micrometres.
Question

Difference between resolution and magnification?

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Answer

Magnification is how many times larger the image is; resolution is the ability to distinguish two points as separate.

Hint: Electron microscopes win on resolution because of the shorter wavelength of electrons.
Question

Three structures in prokaryotes not in eukaryotes.

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Answer

Circular DNA (nucleoid), 70S ribosomes, peptidoglycan cell wall (also plasmids, mesosomes).

Question

Function of the rough endoplasmic reticulum?

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Answer

Ribosomes on its surface synthesise proteins, which are transported and modified inside the RER.

Hint: Follow the route: RER to Golgi to vesicle to membrane.
Question

Role of the Golgi body.

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Answer

Modifies, packages and sorts proteins and lipids into vesicles for secretion or lysosome formation.

AS: Transport in Plants

AS-Level Cambridge 9700
1 / 4
Question

Define transpiration.

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Answer

The loss of water vapour from the leaves and stems of a plant by evaporation and diffusion through the stomata.

Hint: It is a consequence of gas exchange, not a function.
Question

Explain the cohesion-tension theory.

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Answer

Water evaporates from mesophyll cells, lowering water potential; cohesion between water molecules (hydrogen bonds) and adhesion to xylem walls pull a continuous column of water up under tension.

Hint: Mention hydrogen bonds explicitly.
Question

How is xylem adapted for transport?

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Answer

Dead hollow lignified tubes with no end walls, narrow lumen, pits for lateral movement - low resistance to flow.

Question

State the mass flow hypothesis in phloem.

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Answer

Sucrose is actively loaded at the source, lowering water potential so water enters by osmosis, raising hydrostatic pressure; sap flows to the sink where sucrose is unloaded.

Hint: Source to sink, pressure gradient.

A2: Genetics & Gene Technology

A2-Level Cambridge 9700
1 / 4
Question

What is a gene mutation?

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Answer

A change in the base sequence of DNA - substitution, insertion or deletion.

Hint: Insertion/deletion cause a frameshift; substitution may be silent.
Question

Why can a substitution have no effect?

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Answer

The genetic code is degenerate, so the new triplet may still code for the same amino acid (a silent mutation).

Question

Purpose of PCR?

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Answer

To amplify a small sample of DNA: denaturation 95C, annealing of primers 55-65C, extension by Taq polymerase 72C, repeated in cycles.

Hint: Learn the three temperatures.
Question

How does gel electrophoresis separate DNA?

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Answer

DNA is negatively charged and moves to the anode; shorter fragments travel further through the gel in a given time.

Edexcel Unit 1: Molecules & Transport

AS-Level Edexcel WBI11
1 / 4
Question

Describe the structure of a triglyceride.

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Answer

One glycerol joined to three fatty acids by ester bonds formed in condensation reactions.

Hint: Name the bond: ester.
Question

How does a saturated differ from an unsaturated fatty acid?

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Answer

Saturated has no C=C double bonds; unsaturated has one or more, causing kinks so molecules pack less tightly (liquid at room temperature).

Question

Explain how atheroma leads to a heart attack.

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Answer

Plaque narrows the coronary artery and damages the endothelium, a thrombus forms, blood flow to cardiac muscle is blocked, cells respire anaerobically and die.

Hint: Sequence marks - keep the order.
Question

What is the role of the semi-lunar valves?

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Answer

They prevent backflow of blood from the aorta and pulmonary artery into the ventricles when ventricular pressure falls.

Edexcel Unit 4: Energy & Immunity

A2-Level Edexcel WBI14
1 / 4
Question

Write the balanced equation for photosynthesis.

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Answer

6CO2 + 6H2O -> C6H12O6 + 6O2 (in light, with chlorophyll).

Question

What happens in the light-dependent reaction?

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Answer

Light excites electrons in chlorophyll; photolysis of water gives H+, electrons and O2; ATP and reduced NADP are made.

Hint: Products feed the Calvin cycle.
Question

Describe the role of phagocytes.

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Answer

They engulf pathogens by endocytosis into a phagosome, which fuses with a lysosome so enzymes digest the pathogen.

Question

Difference between active and passive immunity?

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Answer

Active: the body makes its own antibodies and memory cells (long lasting). Passive: antibodies are received ready made (fast but short lived).

Hint: Breast milk and antivenom are passive.

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